99$/KG
89$/KG
Product Detailed Description
liposomal L-Theanine 70% is a premium-grade dietary supplement ingredient delivering L-theanine in an advanced liposomal delivery system. L-Theanine (CAS 3081-61-6), also known as γ-glutamylethylamide, N5-ethyl-L-glutamine, or 5-N-ethyl-L-glutamine, is a unique non-protein amino acid with the molecular formula C₇H₁₄N₂O₃ and a molecular weight of 174.20 g/mol. It is a white, crystalline powder with an off-white to white appearance and a characteristic odor and taste, and is freely soluble in water. L-Theanine is predominantly biosynthesized in tea plants (Camellia sinensis), where it contributes to the characteristic umami and fresh flavor that is highly valued in global tea-drinking culture.
L-Theanine is the primary amino acid responsible for the calming yet alert state associated with tea consumption. Unlike pharmaceutical sedatives, L-theanine promotes relaxation without causing drowsiness or impairing cognitive function. It has been extensively studied for its effects on stress management, cognitive performance, sleep quality, and neuroprotection. Emerging food applications include cognitive-enhancing beverages, plant-based preservatives, and low-sodium umami enhancers.
Conventional L-theanine presents formulation challenges. The compound is highly polar, which limits its passive diffusion across intestinal cell membranes and the blood-brain barrier. It also competes with other large neutral amino acids for transport via shared amino acid transporters, which can limit the fraction of orally administered L-theanine that reaches systemic circulation and the central nervous system.
Liposomal encapsulation provides four critical advantages:
1. Enhanced Absorption and Bioavailability — Liposomal L-theanine is designed to enhance the absorption and effectiveness of theanine. Encapsulated in lipid bilayers, this white to off-white powder delivers theanine directly to cells, maximizing its potential to reduce stress, improve focus, and support relaxation without drowsiness. Liposomal delivery systems bypass the competitive amino acid transport pathway by utilizing lipid-mediated absorption mechanisms.
2. Enhanced Brain Availability — L-Theanine crosses the blood-brain barrier via the L-type amino acid transporter (LAT1), which is shared with other large neutral amino acids. Liposomal encapsulation provides an alternative delivery route that improves permeability and transport efficiency across biological membranes, supporting enhanced central nervous system availability.
3. Controlled Release for Sustained Effect — Liposomal delivery provides a sustained-release profile that maintains steady plasma amino acid levels, supporting balanced neurotransmitter modulation rather than a sharp peak followed by rapid decline. Theanine liposomes prepared by two methods had optimal sizes of 154.8 and 169.0 nm, which can effectively accumulate in target tissues.
4. Water Dispersibility and Formulation Flexibility — Liposomal L-theanine is soluble in water and exhibits excellent dispersibility, making it suitable for a wide range of formulations including capsules, powders, functional beverages, and liquid supplements.
| Field | Details |
|---|---|
| Product Name | Liposomal L-Theanine 70% |
| CAS No. | 3081-61-6 |
| Active Ingredient | L-Theanine (γ-Glutamylethylamide) |
| Assay Method | RP-HPLC-DAD method without pre- or post-column derivatization using a buffer-free mobile phase (chromatographic separation of L-theanine with water, eluting other compounds with water-acetonitrile gradient); analysis time 15 minutes; mean intraday precision 0.94 RSD%, interday precision 0.48%, recovery >96.1%, LOQ = 0.38 mg theanine/g dry tea leaf weight. Alternative: pre-column derivatization with DABS-Cl (4-dimethylaminoazobenzene-4′-sulfonyl chloride) with linearity in the range of 1–100 μg/mL (r² = 0.9996), recovery 93.95%–103.90%, LOD 0.6 mg/kg, LOQ 1.7 mg/kg |
| Purity | ≥ 70% L-theanine (HPLC); ≥ 98% assay available |
| Appearance / Color | White to off-white crystalline powder |
| Odor | Slight characteristic odor |
| Taste | Characteristic umami taste |
| Dosage Form | Liposomal powder, water-dispersible |
| Recommended Dosage | 100–400 mg per day; clinical studies have used 200–450 mg/day for sleep support and 50–900 mg/day across trials |
| Application | Stress management, relaxation without drowsiness, cognitive focus and attention, sleep quality improvement, mood support, neuroprotection, immune function |
| Extract Source | Camellia sinensis (tea plant) leaves; fermentation-derived grades also available; sunflower phospholipid liposomal carrier system |
| Molecular Formula | C₇H₁₄N₂O₃ |
| Molecular Weight | 174.20 g/mol |
| Lead Time | In stock: 3–5 business days; Bulk/custom orders: 2–3 weeks |
| Shipping Method | International Express (FedEx / DHL / UPS); air and sea freight available; worldwide shipping including remote areas |
| Storage | Store away from light and heat in a cool, dry place; keep container tightly sealed. Shelf life: 2 years |
| Packaging | 1 kg/aluminum foil bag with two plastic bags inside; 25 kg/carton or fiber drum with aluminum foil bag inside |
High Purity — Standardized to ≥70% L-theanine by HPLC, with ≥98% assay available for pharmaceutical-grade applications.
Liposomal Enhancement — Lipid bilayer encapsulation enhances the absorption and effectiveness of theanine, delivering it directly to cells and maximizing its potential to reduce stress, improve focus, and support relaxation without drowsiness.
Clinically Validated for Stress Reduction — AlphaWave® L-Theanine supplementation for 28 days was safe and significantly decreased perceived stress and light sleep, improved sleep quality, and enhanced cognitive attention in healthy adults with moderate stress.
Clinically Validated for Sleep Quality — A systematic review of 13 eligible trials (n = 550) demonstrated that supplementation with 200–450 mg/day of L-theanine appears to be a safe and effective way to support healthy sleep in adults, with beneficial effects on sleep latency, maintenance, efficiency, perceived sleep satisfaction, and feelings of refreshment on waking. Doses up to 900 mg daily produced no next-day drowsiness, cognitive impairment, or motor deficits.
Clinically Validated for Cognitive Function — A meta-analysis of randomized controlled trials provides evidence that theanine plus caffeine, and theanine alone, could be beneficial for cognitive and mood outcomes.
Unique Relaxed Alertness Mechanism — L-Theanine modulates glutamate receptors to enhance neuroprotection (BDNF upregulation, cortisol reduction), activates AMPK for anti-obesity effects (lipid oxidation, adiponectin secretion), and activates γδ T cells to bolster immunomodulation.
Water-Soluble — Freely soluble in water; suitable for capsules, tablets, powder drink mixes, functional beverages, and liquid formulations.
Batch Traceability — Complete COA, HPLC chromatogram, and identity data provided for each batch.
| Application | Recommended Usage | Notes |
|---|---|---|
| Capsules / Tablets | 100–200 mg per serving | 1–2 capsules daily, with or without food |
| Functional Beverages | 50–100 mg per 250 mL | Water-soluble; suitable for RTD formats including cognitive-enhancing beverages |
| Powdered Drink Mixes | 100–200 mg per sachet | Blend with caffeine (100–200 mg) for synergistic cognitive and mood benefits |
| Stress Relief Formulas | 100–200 mg per serving | Often combined with GABA or magnesium for enhanced calming support |
| Sleep Support Formulas | 200–400 mg per serving | Take 30–60 minutes before bedtime; 200–450 mg/day clinical range |
| Cognitive Focus Formulas | 100–200 mg per serving | Often combined with L-tyrosine or alpha-GPC for sustained attention |
Clinical studies have evaluated L-theanine across a range of doses. A systematic review of 13 eligible trials (n = 550) identified that supplementation with 200–450 mg/day of L-theanine appears to be a safe and effective way to support healthy sleep in adults. Clinical studies typically use L-theanine at a dosage of 100–400 milligrams per day, with most studies suggesting a daily dose of 100–400 mg. A typical split involves 100–200 mg in the morning or early afternoon, and 100–200 mg later in the day if needed for relaxation or sleep. For stress relief, 50–200 mg before a stressful event is commonly used. For sleep in ADHD, 200 mg twice a day has been used. Doses up to 900 mg daily produced no next-day drowsiness, cognitive impairment, or motor deficits across all studied populations aged 9–57 years. Liposomal formulations may allow for more efficient utilization of lower doses due to enhanced bioavailability.
Stock Orders: 3–5 business days after payment confirmation.
Bulk / Custom Orders: 2–3 weeks, depending on quantity and customization requirements.
Shipping Methods: International Express (FedEx, DHL, UPS), air freight, and sea freight.
Coverage: Worldwide shipping, including remote areas.
L-Theanine Encyclopedia — Scientific Background
L-Theanine (CAS 3081-61-6), also known as γ-glutamylethylamide or N5-ethyl-L-glutamine, is a unique non-protein amino acid with the molecular formula C₇H₁₄N₂O₃ and a molecular weight of 174.20 g/mol. It is a white, crystalline powder with a characteristic umami taste and is freely soluble in water. L-Theanine is predominantly biosynthesized in tea plants (Camellia sinensis), where it was first discovered in 1949 as a component of green tea leaves. It is the primary amino acid responsible for the characteristic umami and fresh flavor of green tea and is found naturally in virtually no other plant source at significant concentrations.
| Property | Value |
|---|---|
| CAS No. | 3081-61-6 |
| Molecular Formula | C₇H₁₄N₂O₃ |
| Molecular Weight | 174.20 g/mol |
| IUPAC Name | (2S)-2-Amino-5-(ethylamino)-5-oxopentanoic acid |
| Synonyms | L-Theanine, γ-Glutamylethylamide, N5-Ethyl-L-glutamine, 5-N-Ethyl-L-glutamine |
| Appearance | White to off-white crystalline powder |
| Solubility | Soluble in water |
| Melting Point | 217–218 °C |
L-Theanine is biosynthesized in tea plants from glutamine and ethylamine via the enzyme L-theanine synthetase. Its content in tea leaves is influenced by the type of tea plant, the region of production, and the climate. The highest concentrations are found in the leaves and buds of Camellia sinensis, particularly in green tea and matcha. Commercial L-theanine is produced through two primary methods:
Extraction from Tea Leaves — Natural extraction from green tea leaves yields L-theanine with high purity. A theanine purity of 90% by mass weight of the purified theanine extract fraction can be achieved using water leaching and purification processes.
Microbial Fermentation — Biotechnological production methods using metabolically engineered strains of Escherichia coli or Corynebacterium glutamicum can achieve product purity of 95.6% through a process involving ceramic membrane filtration, electrodialysis, activated-carbon decolorization, and ethanol crystallization.
L-Theanine modulates glutamate receptors to enhance neuroprotection, with mechanisms including BDNF upregulation and cortisol reduction. It has been shown to cross the blood-brain barrier and modulate central nervous system activity through multiple neurotransmitter systems.
L-Theanine modulates GABA, dopamine, and serotonin pathways, supporting its calming yet alerting effects. Its effects on the central nervous system are the most widely studied, and its ability to promote relaxation without sedation distinguishes it from conventional sedatives.
L-Theanine activates AMPK for anti-obesity effects, including lipid oxidation and adiponectin secretion. This metabolic mechanism expands its potential applications beyond stress management to metabolic health.
L-Theanine activates γδ T cells to bolster immunomodulation, providing a link between this tea amino acid and immune function.
The neuroprotective effects of theanine have been summarized in aspects of stress management, cognitive improvement, and reversal of neural injury. It effectively shortens sleep latency and enhances subjective sleep satisfaction without the adverse effects often associated with pharmaceutical sedatives.
Despite its beneficial effects, native L-theanine faces absorption limitations. The compound is highly polar, which limits passive diffusion across biological membranes. It competes with other large neutral amino acids for transport via the L-type amino acid transporter (LAT1), which is shared with leucine, isoleucine, valine, tryptophan, and tyrosine. This competition can limit the fraction of orally administered L-theanine that reaches systemic circulation and the central nervous system.
These limitations have driven the development of advanced delivery systems, including liposomal encapsulation. Liposomal L-theanine is designed to enhance the absorption and effectiveness of theanine, with lipid bilayer encapsulation delivering theanine directly to cells and maximizing its potential.
Liposomal encapsulation of L-theanine provides a fundamentally different delivery mechanism from conventional forms. The phospholipid bilayer surrounds the L-theanine molecule, forming a protective shell that enhances stability, absorption, and cellular uptake. This delivery system:
Enhances the absorption and effectiveness of theanine
Delivers theanine directly to cells
Bypasses the competitive LNAA transport pathway
Provides a controlled release profile for sustained effects
Theanine liposomes prepared by two methods had optimal sizes of 154.8 and 169.0 nm, which can effectively accumulate in target tissues. A liposomal composition comprising L-theanine has been developed for sublingual application, with the method potentially used in the treatment of stress, tension headaches, attention deficit disorder (ADD), attention deficit disorder hyperactivity (ADHD), claustrophobia, hyperactivity, and anxiety. Liposomal NeuroCalm™ is a unique blend of GABA and L-theanine that uses a liposomal delivery system with pure essential phospholipids from soy lecithin to support mental calm and a healthy response to stress.
A systematic review and meta-analysis published in Nutritional Neuroscience (2026) identified 13 eligible trials (n = 550) that examined the effect of L-theanine (50–900 mg/day) as a standalone intervention on sleep-related outcome measures. The review concluded that supplementation with 200–450 mg/day of L-theanine appears to be a safe and effective way to support healthy sleep in adults. Beneficial effects were reported on both objective and participant-reported outcomes, including measures linked to sleep latency, maintenance and efficiency, perceived sleep satisfaction, and feelings of refreshment and recovery on waking.
A randomized, double-blind, placebo-controlled trial evaluated AlphaWave® L-Theanine supplementation for 28 days in healthy adults with moderate stress. The results demonstrated that L-theanine supplementation was safe and significantly decreased perceived stress and light sleep, improved sleep quality, and enhanced cognitive attention in the studied population.
A systematic review and meta-analysis of randomized controlled trials published in PubMed (2025) evaluated the effects of tea or its bioactive compounds L-theanine or L-theanine plus caffeine on cognition, sleep, and mood in healthy participants. The meta-analysis provides evidence that theanine plus caffeine, and theanine alone, could be beneficial for cognitive and mood outcomes.
A clinical trial is investigating whether L-theanine can help cancer survivors manage stress and improve mood during the surveillance phase of their care. The study uses a dose of 200 mg once daily versus twice daily. L-theanine is particularly promising because previous research in healthy populations has shown it can promote relaxation without causing drowsiness and reduce stress responses.
L-Theanine is generally well tolerated with an excellent safety profile. Doses up to 900 mg daily produced no next-day drowsiness, cognitive impairment, or motor deficits across all studied populations aged 9–57 years. L-Theanine does not cause the sedation, cognitive impairment, or dependence associated with pharmaceutical sedatives.
Regulatory status varies by region:
United States — L-Theanine is regulated as a dietary supplement ingredient and is Generally Recognized As Safe (GRAS) for use in food and beverages. It is available in the United States as a dietary supplement and functional food ingredient.
European Union — L-Theanine is used as a food supplement ingredient in EU member states, with regulatory status varying by country.
Japan — L-Theanine is approved for use in food and beverages and is widely consumed in functional foods.
Global Standards — L-Theanine is monographed in the USP and other pharmacopoeias. It is widely commercialized as a dietary supplement ingredient, food additive, and functional food ingredient.
Testing protocols must adhere to international standards including USP, European Pharmacopoeia, and FAO/WHO Codex Alimentarius. Formulators should verify local regulatory requirements prior to market entry.
This product is intended for research, nutraceutical, and functional food applications. Not intended to diagnose, treat, cure, or prevent any disease.
99$/KG
89$/KG
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